Application of Weighted Gene Co-expression Network.
We examined the manually curated gene sets in context of an atlas of gene expression of many normal human tissues. To refine gene sets, we clustered the genes in each set based on co-expression across the tissues to produce more tightly co-regulated children gene sets that are also likely more accurate representations of the biological process or processes described by the gene set.
Gene expression is the process by which specific genes are activated to produce a required protein. An organism's phenotype is determined by the proteins produced, depending on environmental.
Gene set enrichment analysis (GSEA) is a statistical method to determine if predefined sets of genes are differentially expressed in different phenotypes. Predefined gene sets may be genes in a.
PLANEX shows Pearson’s correlation coefficients (PCCs; r-value) that distribute from a gene of interest for a given microarray platform set corresponding to a particular organism. To support PCCs, PLANEX performs an enrichment test of Gene Ontology terms and Cohen’s Kappa value to compare functional similarity for all genes in the co-expression database.
WGCNA: Weighted gene co-expression network analysis. This code has been adapted from the tutorials available at WGCNA website. For the installation and more detailed analysis, please visit the website. Getting started: in order to run R on Orchestra, we will first connect to an interactive queue.
Differential expression analysis means taking the normalised read count data and performing statistical analysis to discover quantitative changes in expression levels between experimental groups. For example, we use statistical testing to decide whether, for a given gene, an observed difference in read counts is significant, that is, whether it is greater than what would be expected just due.
The movie is set in 1927 and it takes a look at Hollywood’s reaction when the sound was first introduced and used into the film production industry. In the movie, the opening sequence began with Gene, Donald and Debbie dressed in yellow raincoat, singing and dancing the song “Singing in the Rain”.